Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2022 , Vol 28 , Issue 2
Effects of Melatonin Addition to the Cold Storage Medium on Cumulus Oocyte Complex Apoptosis, Viability and In Vitro Maturation Rates of Cat Oocytes
Ramazan ARICI1, Kemal AK1, Serhat PABUCCUOĞLU1, Sema BİRLER1, Kamber DEMİR1, Selin YAĞCIOĞLU1, Ahmet ESER1, Nur ERSOY1, İdil ORUÇ1, Gül BAKIRER ÖZTÜRK2, Evrim KÖMÜRCÜ BAYRAK3, Bilge ÖZSAİT SELÇUK3, Andaç KILIÇKAP4, Mithat EVECEN1
1Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University-Cerrahpaşa, TR-34320 Avcılar, İstanbul - TÜRKİYE
2Department of Laboratory Animals Biology, Institute of Aziz Sancar Experimental Medicine, Istanbul University, TR-34393 Fatih, İstanbul - TÜRKİYE
3Department of Genetic, Institute of Aziz Sancar Experimental Medicine, Istanbul University, TR-34393 Fatih, İstanbul - TÜRKİYE
4Istanbul Medeniyet University, Science and Advanced Technologies Research Center (BILTAM), TR-34700 İstanbul - TÜRKİYE
DOI : 10.9775/kvfd.2021.26659 Usage of oocytes obtained from ovaries aft er long-term cold storage for in vitro embryo production is a promising tool for the protection of wildlife and endangered animal species. Mammalian oocytes are susceptible to oxidative stress with regard to the high lipid content of plasma membranes. Melatonin is known as a powerful antioxidant and anti-apoptotic agent due to its ability to eliminate toxic oxygen derivatives and reduce the formation of reactive species. Th is study was performed to verify the optimal environmental conditions for long-term preservation of cat ovaries (Felis domesticus) by adding diff erent concentrations of melatonin (500, 750 and 1000 μM) to the storage medium (0.9% NaCl) as an antioxidant to be preserved at 4°C for 24 h. To determine the eff ect of melatonin on cat oocytes collected from stored ovaries, the anti and proapoptotic gene levels in cumulus oophorus, the in vitro maturation rates, the cell membrane and oocytes viability were evaluated. In all melatonin added groups regardless of whether they are stored in the cold; Pro-apoptotic gene levels (BAX) were determined to be upregulated however, antiapoptotic gene levels (BCL-2) were downregulated in cumulus cells (P<0.05). Th e cell membrane stability and cell viability rates of oocytes began to deteriorate in parallel with the rate of melatonin increase. In parallel with these findings, in vitro maturation rates of oocytes were negatively aff ected as the amount of melatonin increased (P≤0.001). In conclusion the results showed that adding melatonin (500,750 or 1000 μM) to the ovarian transport and storage medium had negative eff ect on in vitro maturation rate, viability and cell membrane structure of cat oocytes. Keywords : Soğukta saklama, Melatonin, Kedi ovaryumları, İn vitro maturasyon, Apoptozis, Bax, Bcl-2