Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2022 , Vol 28 , Issue 2
The Effect of Total Mix Ration with Xylitol Supplementation on In Vitro Ruminal Total Gas and Methane Production, Digestion Values, Organic Acids, Ammonia-Nitrogen, and the Number of Total Protozoa in Dairy Cattle
Yıldıray SOYLU1, Kanber KARA2, Süleyman Ercüment ÖNEL3, Sena YILMAZ2, Mehmet Akif ÖZTAŞ2, Öznur ASLAN1
1Erciyes University, Faculty of Veterinary Medicine, Department of Internal Medicine, TR-38280 Kayseri - TÜRKİYE
2Erciyes University, Faculty of Veterinary Medicine, Department of Animal Nutrition and Nutritional Diseases, TR-38280 Kayseri - TÜRKİYE
3Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Animal Nutrition and Nutritional Diseases, TR-31006 Hatay - TÜRKİYE
DOI : 10.9775/kvfd.2021.26614 Th e study aimed to determine the eff ect of xylitol, added to the dry period total mix ration (TMR) of dairy cattle, on in vitro rumen fermentation, total gas production, methane production, estimated digestion values, organic acids and ammonia-nitrogen contents and the number of total protozoa. Xylitol was added to dairy cattle TMR at diff erent rates (0%, 2% 4%, and 8%; dry matter basis). Th e xylitol supplementations at 2 and 4% did not aff ect in vitro total gas production, in vitro methane production, metabolizable energy (ME) and organic matter digestibility (OMd) values (P>0.05). However, 8% xylitol supplementation decreased in vitro total gas production, in vitro methane production, ME and OMd (P<0.05). Th e molarities of total volatile fatty acid (TVFA) percentages of acetic acid (AA), propionic acid (PA) and butyric acid (BA) in TVFA, ammonia-nitrogen concentration, and the number of total ciliate protozoa of the in vitro rumen fl uid of xylitol supplementations at 2%, 4%, and 8% were similar to those of control TMR (P>0.05). Th e iso-valeric acid (IVA), iso-butyric acid (IBA) and valeric acid (VA) percentages in TVFA of in vitro rumen fl uid linearly decreased with xylitol supplementation, especially 8% xylitol supplementation (P<0.05). Besides, 2% and 4% xylitol supplementations to dairy cattle TMR numerically increased the concentration of ammonia-nitrogen and the number of total ciliate protozoa in in vitro fermentation fl uid (P>0.05). Consequently, the supplementation of 2% and 4% xylitol to dairy cattle ration did not aff ect the in vitro rumen fermentation (total gas production, methane production, estimated digestion values, organic acids and ammonia-nitrogen parameters). However, 8% supplementation xylitol to dairy cattle ration had the potential to aff ect the before-mentioned in vitro ruminal fermentation parameters adversely. Keywords : Dairy cattle, In vitro gas production, Rumen fermentation, Xylitol